About one-in-six people in the Division of New England got a pay cut on Friday.
Some 9,644 people in the Federal electorate of New England received JobSeeker in September.
Including youth allowance, student benefits, parenting payments, the widow allowance and more, nearly 16,200 received the coronavirus supplement, a $550 top-up to their fortnightly income.
On Friday the supplement was slashed by $300.
The figures, which are based on Parliamentary Library data and were shared with the Leader by the Labor Party, showed the cut would cost the New England region nearly $5 million a fortnight.
The supplement is set to be cut by an additional $250 by the end of the year, to zero.
Unemployed people will earn just $40 a day.
The statistics show regional unemployment has increased by 3,124 people since December 2019, before the COVID-19 crisis.
Senator Tim Ayres said the worst recession since the Great Depression was the worst possible time to cut income support.
"Cuts to JobKeeper and JobSeeker in the same week are a double hit to regional economies like the New England," he said.
"Reducing the JobKeeper rate will not create jobs - it will kill jobs and job opportunities. Cutting JobSeeker to $40 a day will not create jobs - it will just make it hard for the unemployed Australians to sustain themselves in the hunt for work."