Riverina MP Michael McCormack has defeated New England MP Barnaby Joyce in a vote for leadership of the National Party and the Deputy Prime Minister role following a spill motion on Tuesday morning.
Nationals whip Damian Drum declined to provide details on how many votes each candidate secured in the Party Room meeting of 21 Nationals MPs and Senators.
Mr McCormack said it was "a time to rebuild" and not a time to "tear down" before attending a parliamentary church service on Tuesday morning prior to the vote.
Mr Joyce said the National Party was losing its support in regional Australia and two years before the next election was the best time for a leadership change.
Nationals MPs and Senators had been due to choose a replacement for Senator Bridget McKenzie as deputy leader but both leadership roles ended up being put to a vote.
Mr McCormack claimed on Monday morning that there would be no leadership spill after Mr Joyce said he would stand as a candidate if another National moved a motion to declare the party leader role vacant.
On Monday afternoon, Queensland backbench MP Llew O'Brien said he would move a spill motion.
Mr McCormack became Deputy Prime Minister in February 2018 after Mr Joyce stepped down amidst revelations of his affair with a staff member and sexual harassment allegations.
Under Mr McCormack's leadership, the Nationals maintained their representation in the lower house with no net loss of seats at the 2019 federal election.
The Nationals gained one seat in the 2016 election, which took place five months after Mr Joyce took aver the leadership from Warren Truss.