The Queensland Long Ride saw between 80 and 100 Queenslanders jump on their bikes as part of the route to Uluru.

Tamworth was the riders' first stop after starting out from Amberley in Queensland on Sunday morning.

Queensland Long Ride co-ordinator Terry Lubomirski said they expected to raise as much as $380,000 for the Prostate Cancer Foundation of Australia for research, providing information and family support.

"It's not just the person who has got the cancer who needs financial support," he said.

He said often a spouse or relative would have to leave their work to care for the cancer patient, adding to the monetary strain.

Mr Lubomirski said the riders had been warmly welcomed by Tamworth locals, who discussed the devastating cancer with them and made donations, with one generous local handing over $100 to the cause on the spot.

He said the long ride was not only about raising money but also about raising awareness.

He said they had encountered a man in his 60s on a previous ride and convinced him to get checked for prostate cancer.

"It turned out he had it and he's still alive today. Early diagnosis is so important," Mr Lubomirski said.

"I haven't lost any friends to it, thank goodness, but I have five or six who have been diagnosed.

"It's pretty tough to ride 500 to 600 kilometres a day on a bike, but the way we think of it is, it's nothing like what prostate cancer sufferers go through."